Combination of immune stimulating adjuvants with poly(lactide-co-glycolide) microspheres enhances the immune response of vaccines

► In this study we have evaluated the immunogenicity of PLGA microspheres encapsulating BSA and different immunostimulatory adjuvants. ► Microspheres sized around 1 μm and released the peptide showing the typical triphasic profile. ► All the microspheres developed high antibody responses, which were IgG1/IgG2a balanced, when administered to Balb/c mice. ► Only combination of MPLA and α-galactosylceramide within the microspheres increased the cellular immune response.