Delivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis

► We utilize novel vaccine technology (SAVINE) for Mycobacterium tuberculosis. ► We deliver multiple antigens as single polypeptide via DNA and viral vectors. ► We demonstrate immune responses in C57BL/6 and BALB/c mice. ► We demonstrate protection in a murine model of tuberculosis with DNA-Savine. ► Co-delivery of TB and HIV-Savinesstimulate effective T-cell responses to each.