کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10969899 1102996 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ΔznuAΔpurE Brucella abortus 2308 mutant as a live vaccine candidate
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
ΔznuAΔpurE Brucella abortus 2308 mutant as a live vaccine candidate
چکیده انگلیسی
To create a new, safe brucellosis live vaccine, a double mutant strain was constructed from Brucella abortus 2308. Using the ΔznuA B. abortus 2308 mutant, a second mutation was introduced by deleting purE gene. The ΔznuA ΔpurE B. abortus 2308 strain was less capable of surviving in macrophages. When evaluated in vivo, it was cleared within 8 weeks (wks) from mice, causing significantly less inflammation than spleens obtained from wild-type B. abortus 2308-infected mice. Furthermore, two doses of ΔznuA ΔpurE B. abortus 2308 conferred 0.79 log protection, similar to S19 as did a single dose of ΔznuA B. abortus 2308. Thus, this study shows the ΔznuA ΔpurE B. abortus 2308 strain to be a potential livestock vaccine candidate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 28, Issue 4, 22 January 2010, Pages 1069-1074
نویسندگان
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