کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10970348 | 1103068 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of different genes in the CS6 operon for surface expression of Enterotoxigenic Escherichia coli colonization factor CS6
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کلمات کلیدی
PBSmAbETECMonoclonal antibody - آنتی بادی مونوکلونالheat-labile enterotoxin - انتروتوکسین گرم لابلیheat-stable enterotoxin - انتروتوکسین گرم پایدارColonization factor - فاکتور کلونیزاسیونPhosphate buffered saline - فسفات بافر شورpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Role of different genes in the CS6 operon for surface expression of Enterotoxigenic Escherichia coli colonization factor CS6 Role of different genes in the CS6 operon for surface expression of Enterotoxigenic Escherichia coli colonization factor CS6](/preview/png/10970348.png)
چکیده انگلیسی
Coli surface antigen 6, CS6, is one of the most prevalent colonization factors (CFs) associated with Enterotoxigenic Escherichia coli (ETEC) bacteria, the most common cause of diarrhea among infants and children in developing countries. The CS6 operon encodes two structural subunit proteins, CssA and CssB, a chaperon, CssC, and an usher, CssD. Since little is known about the relationship of the genes and their role in expression and surface assembly of CS6, the operon was cloned into a laboratory E. coli strain, and mutants were constructed by creating deletions in each of the genes. Examination of protein expression by different methods, using monoclonal antibodies with specificities for the individual CS6 structural proteins, suggested that the usher (CssD) was not involved in assembly or surface expression of CS6 whereas deletion of the chaperon (CssC) significantly reduced levels of CssA, but not of CssB. Binding studies with CaCo-2 cells demonstrated that there is a combined effect of CssC and CssD on receptor binding, presumably associated with their activities in assembly and transport of the structural subunits. These findings may have important implications for the construction of strains expressing high levels of CS6 on the surface for eventual use in an ETEC vaccine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 26, Issue 42, 3 October 2008, Pages 5373-5380
Journal: Vaccine - Volume 26, Issue 42, 3 October 2008, Pages 5373-5380
نویسندگان
Joshua Tobias, Michael Lebens, Susanne KällgÃ¥rd, Matilda Nicklasson, Ann-Mari Svennerholm,