کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971548 | 1106477 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell type-specific differences in β-glucan recognition and signalling in porcine innate immune cells
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کلمات کلیدی
Limulus Amebocyte LysateCR3LALRLUmAbsFAKMDMDCsSmall interfering RNA - RNA تداخل کوچکROS - ROSsiRNA - siRNAβ-glucans - β-گلوکانSyk - بیمارPig - خوکDectin-1 - دیکتین-1Innate immune cells - سلول های ایمنی بدن انسانDendritic cells - سلول های دندریتیکspleen tyrosine kinase - طرز تیروزین کینازMAMPS - مامس هاmonocyte-derived macrophages - ماکروفاژهای حاصل از مونوسیتrelative light units - واحدهای نسبی نورMonoclonal antibodies - پادتنهای تَکتیرهPropidium iodide - پروتئین یدیدquantification cycle - چرخه محاسبهfocal adhesion kinase - کیناز چسبندگی کانونیReactive oxygen species - گونههای فعال اکسیژنComplement receptor 3 - گیرنده تکمیلی 3
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
β-glucans exert receptor-mediated immunomodulating activities, including oxidative burst activity and cytokine secretion. The role of the β-glucan receptors dectin-1 and complement receptor 3 (CR3) in the response of immune cells towards β-glucans is still unresolved. Dectin-1 is considered as the main β-glucan receptor in mice, while recent studies in man show that CR3 is more important in β-glucan-mediated responses. This incited us to elucidate which receptor contributes to the response of innate immune cells towards particulate β-glucans in pigs as the latter might serve as a better model for man. Our results show an important role of CR3 in β-glucan recognition, as blocking this receptor strongly reduced the phagocytosis of β-glucans and the β-glucan-induced ROS production by porcine neutrophils. Conversely, dectin-1 does not seem to play a major role in β-glucan recognition in neutrophils. However, recognition of β-glucans appeared cell type-specific as both dectin-1 and CR3 are involved in the β-glucan-mediated responses in pig macrophages. Moreover, CR3 signalling through focal adhesion kinase (FAK) was indispensable for β-glucan-mediated ROS production and cytokine production in neutrophils and macrophages, while the Syk-dependent pathway was only partly involved in these responses. We may conclude that CR3 plays a cardinal role in β-glucan signalling in porcine neutrophils, while macrophages use a more diverse receptor array to detect and respond towards β-glucans. Nonetheless, FAK acts as a master switch that regulates β-glucan-mediated responses in neutrophils as well as macrophages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 48, Issue 1, January 2015, Pages 192-203
Journal: Developmental & Comparative Immunology - Volume 48, Issue 1, January 2015, Pages 192-203
نویسندگان
Kim Baert, Eva Sonck, Bruno M. Goddeeris, Bert Devriendt, Eric Cox,