کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971702 | 1106514 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene cloning of a sigma class glutathione S-transferase from abalone (Haliotis diversicolor) and expression analysis upon bacterial challenge
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کلمات کلیدی
LPSqPCRpgdssqRT-PCRCDNBSSHORF3-methylcholanthreneGSTRT-PCRHLS1-chloro-2,4-dinitrobenzene - 1-کلرو-2،4-دینیتروبنزنCDD - CSDHaliotis diversicolor - Haliotis varicolor3-MC - MC-3ROS - ROSDifferential gene expression - بیان ژن دیفرانسیلrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدconserved domain - دامنه محافظت شدهreverse transcription PCR - رونویسی معکوس PCRSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازopen reading frame - قاب خواندن بازlipopolysaccharide - لیپوپلی ساکاریدRace - مسابقهSuppression subtractive hybridization - هیبریداسیون تفکیک پذیری سرکوبquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPCR - واکنش زنجیرهٔ پلیمرازMolecular weight - وزن مولکولیconserved domain database - پایگاه داده حوزه حفظ شدهProstaglandin D2 synthase - پروستاگلاندین D2 سنتازneighbor-joining - پیوستن همسایهBacterial challenge - چالش باکتریاییglutathione S-transferases - گلوتاتیون S-transferasesglutathione S-transferase - گلوتاتیون S-ترانسفرازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glutathione S-transferases (GSTs) are a multigene family of xenobiotic metabolizing phase II detoxification enzymes which take part in many pathological and physiological processes, and which can potentially be used as indicators and biomarkers for cancer diagnoses and organic or inorganic pollutant exposure. In this study, a full-length cDNA of a sigma class GST (abGSTsigma) (GenBank accession number EF546619) from variously colored abalone (Haliotis diversicolor) was identified. It was 1328Â bp containing an open reading frame of 624Â bp, encoding 208 amino acid residues with a predicted protein molecular weight of 23.67Â kDa and an estimated pI of 5.67. Sequence analysis showed that the predicted protein sequence of abGSTsigma cDNA contained the conserved domain of the GST_N_Sigma_like (PSSM: cd03039) and GST_C_Sigma_like (PSSM: cd03192). Alignment analysis demonstrated that the abGSTsigma of H. diversicolor was in a branch position with other known class sigma GSTs from different organisms. The abGSTsigma mRNA was distributed in multiple tissues tested and was highly demonstrated in the gill and mantle of normal abalones. In bacteria-challenged abalone, the abGSTsigma gene was significantly expressed in the hemocytes, gill, mantle and digestive gland and the total GSTs enzyme and SOD were also induced in the four tissues. The increased activities of SOD and GSTs can result in the elimination of reactive oxygen species (ROS) indicating antioxidant activities involved. The preliminary work revealed that the sigma class glutathione S-transferase gene abGSTsigma, a phase II detoxification enzyme, had a positive response to bacterial challenge, and that will lead to an insightful study on elucidating the interactions between immune responses and biotransformation exerted by abGSTsigma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 33, Issue 9, September 2009, Pages 980-990
Journal: Developmental & Comparative Immunology - Volume 33, Issue 9, September 2009, Pages 980-990
نویسندگان
Hong-Lin Ren, Dan-Dan Xu, Singaram Gopalakrishnan, Kun Qiao, Wei-Bin Huang, Ke-Jian Wang,