کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10972904 1107349 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparative enzymatic synthesis of trypanothione and trypanothione analogues
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Preparative enzymatic synthesis of trypanothione and trypanothione analogues
چکیده انگلیسی
Trypanosomatids, the causative agents of several tropical diseases, have a unique thiol metabolism based on trypanothione [bis(glutathionyl)spermidine]. Enzymes of the pathway are attractive drug target molecules but the availability of trypanothione remains an obstacle. Here, we present a convenient method for the production of trypanothione and trypanothione disulfide in >200 mg quantities using a mutant of Crithidia fasciculata trypanothione synthetase in which Cys59 has been replaced by an alanine residue. The reagent costs less than 1% of the commercial price of trypanothione disulfide. The protocol also allows the synthesis of related glutathione conjugates. It will greatly facilitate the thorough analysis of this parasite's metabolism and drug screening approaches against trypanothione-dependent enzymes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 39, Issue 10, August 2009, Pages 1059-1062
نویسندگان
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