کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11000149 1422141 2018 34 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Potential highly polymorphic short tandem repeat markers for enhanced forensic identity testing
ترجمه فارسی عنوان
نشانگرهای تکرارپذیری کوتاه مدت چندجملهای بسیار بالقوه برای تست هویت سنی عینی را افزایش می دهد
کلمات کلیدی
تاندون کوتاه مدت تکرار می شود تنوع توالی، پلیمورفیسم، شناسایی قانونی، توالی انعطاف پذیری موازی،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی
Due to their polymorphic nature, short tandem repeats (STRs) are well-studied and routinely used genetic markers for forensic DNA typing. However, even the largest STR multiplexes are limited in their ability to parse out individuals in a DNA mixture sample, due to alleles shared by size detected by capillary electrophoresis and challenges in resolving minor alleles from stutter, and inherent heterozygote imbalance. In this study, STRs were explored in public datasets that displayed sequence variation and may have limited allele length spread. STRs were first selected using fundamental criteria of high heterozygosity, tetra-, penta-, or hexanucleotide repeat length, and overall relative narrow allele spread (based on length). All candidates were further scrutinized for chemistry compatibility. The resulting STRs were multiplexed and sequenced by massively parallel sequencing in a limited sample population set. Each candidate STR was evaluated for analytical performance and desired biological properties. The findings presented describe a refined set of 53 potential highly polymorphic STR markers (high sequence diversity and heterozygosity; reduced allele spread) that may be suitable to supplement the current core marker set(s) for possible enhanced characterization of complex DNA profiles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 37, November 2018, Pages 162-171
نویسندگان
, , ,