کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11016073 | 1781692 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish
ترجمه فارسی عنوان
استخراج آنزیمی و کروماتوگرافی مایع - اسپکترومتری جرمی پلاسما همراه با القایی برای تعیین گونه های آرسنیک در ماهی
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کلمات کلیدی
MMAIARCArsenocholineKEDArsenobetaineROXASCHPLC-ICP-MSDMAASAInternational Agency for Research on Cancer - آژانس بین المللی تحقیقات سرطانEuropean Union - اتحادیه اروپاdimethylarsinic acid - اسید دی متیلارسینیکmonomethylarsonic acid - اسید مونومیتیلارسونیکKinetic energy discrimination - تبعیض انرژی جنبشیDetection limit - حد تشخیصNIT - شبfood and agricultural organization - غذا و سازمان کشاورزیFAO - فائوASB - لطفاFish - ماهیQuantification limit - محدودیت محدودهNAPP - نیشHelium - هلیمProtease - پروتئازAmmonium carbonate - کربنات آمونیومArsenic species - گونه های آرسنیک
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A sensitive, simple and rapid method for the simultaneous determination of eleven arsenic species has been developed. A high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) technique was used for the analysis of eleven arsenic species in less than 17âmin. Different extraction solutions were explored and the recovery results using water and aqueous acidic solvents, aqueous organic solvents and enzymes showed that 40âmg protease with 0.75âmL 0.5% hydrochloric acid (v/v) as the extraction agent gave the best experimental results. Species separation with ammonium carbonate solution as the mobile phase was conducted on an anion-exchange chromatographic column using gradient elution. The column temperature was 20â°C and kinetic energy discrimination (KED) was employed to eliminate spectral interference. The use of KED mode effectively removed interference from 75ArCl. The detection limit (LD) of the method was in the range of 0.11-0.59âμg kgâ1. Repeatability values obtained for spiked real fish samples were in the range of 1.1%-7.6%. Accuracy was calculated based on the analysis of spiked real fish samples at five concentration levels. Obtained recoveries were 91%-106%. The validated method was used in a pilot study to analyze real samples of fish, the organic arsenic especially AsB was the major arsenic specie present in the analyzed samples, only trace amount of inorganic arsenic were detected. The analytical method should improve the assessment of human exposure associated with arsenic intake from fish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1573, 26 October 2018, Pages 48-58
Journal: Journal of Chromatography A - Volume 1573, 26 October 2018, Pages 48-58
نویسندگان
Fa Zhao, Yanming Liu, Xiqi Zhang, Rui Dong, Wenjiang Yu, Yanfang Liu, Zhimou Guo, Xinmiao Liang, Jianhua Zhu,