کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11016074 | 1781692 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation and application of a restricted access material with hybrid poly(glycerol mono-methacrylate) and cross-linked bovine serum albumin as hydrophilic out layers for directly on-line high performance liquid chromatography analysis of enrofloxacin a
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A novel restricted access material (RAM) was prepared through combination of hydrophilic polymer poly(glycerol mono-methacrylate) and cross-linking bovine serum albumin (BSA) for direct biological analysis. In the material preparation, the poly(styrene-co-divinylbenzene) and poly(glycerol mono-methacrylate) were grafted on silica successively by atom transfer radical polymerization. Then the BSA was adsorbed on the material and cross-linked through an in-column process. The BSA recoveries of the resulted RAM were higher than 99.3%. Small molecules such as alkylbenzene and quinolone antibiotics could be retained with a reversed phase mechanism. The column packed with RAM had good long term stability and could last at least five months without significantly changed in its efficiencies. An on-line SPE/HPLC method for the analysis of enrofloxacin and gatifloxacin in milk samples was established by using the resulted RAM as the solid-phase extraction material. The detection and quantitation limits of two antibiotics were 8.22 and 27.4ângâmLâ1, respectively. In the method validation, least recovery 88.5% with relative standard deviation of less than 3.9% was obtained. It demonstrated that the method is reliable and the material can be used for the direct analysis of veterinary drug residues in biological samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1573, 26 October 2018, Pages 59-65
Journal: Journal of Chromatography A - Volume 1573, 26 October 2018, Pages 59-65
نویسندگان
Wei Huang, Panpan Wang, Ping Jiang, Xiangchao Dong, Shen Lin,