کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11025921 | 1666476 | 2019 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Environmental exposure of humans to bromide in the Dead Sea area: Measurement of genotoxicy and apoptosis biomarkers
ترجمه فارسی عنوان
قرار گرفتن در معرض محیط زیست از انسان به برومید در ناحیه دریای مرده: اندازه گیری زیستی ژنوتوکسید و آپوپتوز
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کلمات کلیدی
NMPTCTPPBSLMPBSA - BSAbovine serum albumin - آلبومین سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدbromine - برمELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاApoptosis - خزان یاختهایCNS - دستگاه عصبی مرکزیComet assay - روش کامت یا روش سنجش ستاره دنبالهدار Genotoxicity - سمیت ژنتیکیcentral nervous system - سیستم عصبی مرکزیphosphate buffer saline - فسفات بافر شورBiomarkers - نشانگر زیستی یا بیومارکرNormal melting point - نقطه ذوب طبیعیlow melting point - نقطه ذوب پایینTranslationally controlled tumor protein - پروتئین تومور پروتئینی کنترل شده با پروتئین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Bromide (Brâ) is a bromine atom with a negative charge which is released mainly in the production of pesticides and flame retardants. It is also found naturally in seawater. Br¯ has been associated with many detrimental effects such as respiratory problems, gastric hemorrhages, and dermal burns. The aim of the study was to monitor serum bromide in humans and to correlate its level with genotoxicity and apoptosis in human. The study utilized comet assay, to measure DNA damage in peripheral leukocytes (i.e. T%DNA), enzyme-linked immunosorbent assay were used to determine fortilin level as an apoptosis marker, and spectrophotometry to measure serum Br¯ in two populations at the Dead Sea area, which are located close to and far from a local bromine factory: Ghor As-safi and Deir Alla, respectively. The biomarkers were compared with the correlating serum Br¯. A total of 397 individuals were involved in the study. The serum Brâ and the genotoxicity biomarker were significantly higher (pâ<â 0.001) in Ghor As-safi than in Deir Alla. In contrast, serum fortilin did not differ significantly between the two regions (pâ>â 0.05). T%DNA was significantly correlated (râ=â0.867, pâ<â 0.01) to serum Br¯. In conclusion, residing near a bromide source site is increasing the bromide body burden, and enhancing genotoxicity with no detectible apoptosis. Furthermore, the selected biomarkers could serve as tools to assess the toxicity of bromide as a consequence of environmental exposure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 837, January 2019, Pages 34-41
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 837, January 2019, Pages 34-41
نویسندگان
Shreen Deeb Nusair, Mohammad Jamil Almasaleekh, Hasan Abder-Rahman, Mohammad Alkhatatbeh,