کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11033766 | 1570071 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and evolution analysis of Wt1 and StAR genes in Andrias davidianus
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کلمات کلیدی
DAX1qRT-PCRWT1DDSWNT4SF-1Andrias davidianus - آندریاس داودیانSex differentiation - تمایز جنسیتیrapid-amplification of cDNA ends - تکثیر سریع cDNA به پایان می رسدStar - ستارهDenys-Drash syndrome - سندرم دنیس-دراشSry - سوریEvolution - فرگشت HPG axis - محور HPGRace - مسابقهquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استmis - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Wt1 and StAR are two multifunctional genes that play an important role in sexual differentiation, especially in development of testicles and spermatogenesis, but the studies of amphibians are rare. In this study, we have cloned and characterized the complete cDNA of Wt1 and StAR in Andrias davidianus. The Wt1 has two isoforms, encoding 416 aa or 419 aa protein with a conserved zinc finger structure region. The StAR encodes 287 aa protein with a conserved domain containing putative lipid binding sites. The two genes showed high homology among amphibians by phylogenetic analysis. Molecular evolution analysis indicated that Wt1 gene underwent positive selection, while StAR gene showed no positive selection. Tissue expression patterns revealed that Wt1 expression was higher in liver, moderate in testis and kidney, low in the rest of the tissues, while StAR expression was higher in testis, moderate in kidney and brain, low in the rest of the tissues. Moreover, compared to female and reversal-female, Wt1 and StAR both showed higher expression level in male and reversal-male. Our results suggest the important role of Wt1 and StAR in sexual development of Andrias davidianus, and also provide information for functional study of amphibian Wt1 and StAR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 13, December 2018, Pages 158-165
Journal: Gene Reports - Volume 13, December 2018, Pages 158-165
نویسندگان
Quanhe Wang, Haifeng Tian, Yan Meng, Hanbing Xiao, Wei Li, Qiaomu Hu,