کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11262708 | 1840478 | 2019 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA profile analysis for discrimination of monozygotic twins using massively parallel sequencing and real-time PCR
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
In general, it is extremely problematic to discriminate between monozygotic twins (MZTs), who share the same genomic DNA sequence, using traditional DNA-based identification methods such as short tandem repeat profiling. MicroRNAs (miRNAs) have shown potential in forensic applications owing to their low molecular weight, abundant and tissue-specific expression. In this study, we utilized massively parallel sequencing technology to perform genome-wide profiling of miRNAs in the blood from four pairs of healthy MZTs. On average, 158 miRNAs were detected in each individual and 14% of which were differentially expressed within each pair of MZTs. The miRNAs with the most significant differences in expression between the twins were confirmed using real-time polymerase chain reaction. Our results demonstrated that miRNAs have potential for use as molecular markers in MZTs discrimination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 38, January 2019, Pages 23-31
Journal: Forensic Science International: Genetics - Volume 38, January 2019, Pages 23-31
نویسندگان
Chen Fang, Jing Zhao, Xu Liu, Jingjing Zhang, Yunwang Cao, Yaran Yang, Chunrui Yu, Xiaoli Zhang, Jialin Qian, Wenli Liu, Huijuan Wu, Jiangwei Yan,