کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163401 1490941 2015 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Orbitrap technology for comprehensive metabolite-based liquid chromatographic–high resolution-tandem mass spectrometric urine drug screening – Exemplified for cardiovascular drugs
ترجمه فارسی عنوان
تکنولوژی اوربیوتر برای کروماتوگرافی مایع مبتنی بر متابولیت های جامد، ایزوله با وضوح بالا و انبساط طیف سنجی ادراری نمونه ای برای داروهای قلبی عروقی است
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• First study on the application of Orbitrap technology for comprehensive drug screening in clinical and forensic toxicology.
• Simple workup, sufficient separation, and powerful screening and identification using modern high resolution MS.
• Validation of the assay according to guidelines for qualitative approaches.
• Elucidation of the power of new data evaluation software in combination with a new reference drug and metabolite library.
• Great relevance for science and practice in clinical and forensic toxicology.

LC–high resolution (HR)-MS well established in proteomics has become more and more important in bioanalysis of small molecules over the last few years. Its high selectivity and specificity provide best prerequisites for its use in broad screening approaches. Therefore, Orbitrap technology was tested for developing a general metabolite-based LC–HR-MS/MS screening approach for urinalysis of drugs necessary in clinical and forensic toxicology. After simple urine precipitation, the drugs and their metabolites were separated within 10 min and detected by a Q-Exactive mass spectrometer in full scan with positive/negative switching, and subsequent data dependent acquisition (DDA) mode. Identification criteria were the presence of accurate precursor ions, isotopic patterns, five most intense fragment ions, and comparison with full HR-MS/MS library spectra. The current library contains over 1900 parent drugs and 1200 metabolites. The method was validated for typical drug representatives and metabolites concerning recovery, matrix effects, process efficiency, and limits showed acceptable results. The applicability was tested first for cardiovascular drugs, which should be screened for in poisoning cases and for medication adherence of hypertension patients. The novel LC–HR-MS/MS method allowed fast, simple, and robust urine screening, particularly for cardiovascular drugs showing the usefulness of Orbitrap technology for drug testing.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 891, 3 September 2015, Pages 221–233
نویسندگان
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