کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163682 1490979 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an analytical methodology using Fourier transform mass spectrometry to discover new structural analogs of wine natural sweeteners
ترجمه فارسی عنوان
توسعه یک روش تحلیلی با استفاده از اسپکترومتری توزیع تبدیل فوریه برای کشف ساختارهای جدید ساختاری شیرین کننده های طبیعی شراب
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• An analytical approach based on HRMS targeted screening was developed to search for structural analogs of sweeteners.
• The purification protocol using CPC and HPLC was guided by HRMS.
• Four new triterpenoids were identified in oak wood, two of them were sweet.
• Structural information on nature, position and sequence of functional groups were provided by HRMSn developments.

Volatile and non-volatile molecules are directly responsible for the thrill and excitement provided by wine-tasting. Their elucidation requires powerful analytical techniques and innovative methodologies. In a recent work, two novel sweet compounds called quercotriterpenosides (QTT) were identified in oak wood used for wine-ageing. The aim of the present study is to discover structural analogs of such natural sweeteners in oak wood. For this purpose, an analytical approach was developed as an alternative to chemical synthesis. Orbitrap mass spectrometry proved to be a crucial technique both to demonstrate the presence of QTT analogs in oak wood by targeted screening and to guide the purification pathway of these molecules using complementary chromatographic tools. Four compounds were isolated and identified for the first time: two isomers, one glucosyl derivative and one galloyl derivative of QTT. Their tasting showed that only the two new isomers were sweet, thus demonstrating both the pertinence of the strategy and the influence of functional groups on gustatory properties. Finally, this paper presents some developments involving multistage Fourier transform mass spectrometry (FTMS) to provide solid structural information on these functional groups prior to any purification of compounds. Such analytical developments could be particularly useful for research on taste-active or bio-active products.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 853, 1 January 2015, Pages 425–434
نویسندگان
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