Solid phase microextraction fills the gap in tissue sampling protocols

• In vivo SPME sampling was used during lung and liver transplantation.
• Selection of the probe, transportation, storage conditions and analyte coverage were discussed.
• Organ preservation procedures were investigated using principal component analysis.

Metabolomics and biomarkers discovery are an integral part of bioanalysis. However, untargeted tissue analysis remains as the bottleneck of such studies due to the invasiveness of sample collection, as well as the laborious and time-consuming sample preparation protocols. In the current study, technology integrating in vivo sampling, sample preparation and global extraction of metabolites – solid phase microextraction was presented and evaluated during liver and lung transplantation in pig model. Sampling approaches, including selection of the probe, transportation, storage conditions and analyte coverage were discussed. The applicability of the method for metabolomics studies was demonstrated during lung transplantation experiments.

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