کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1164364 1491002 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gold nanocatalyst-based immunosensing strategy accompanying catalytic reduction of 4-nitrophenol for sensitive monitoring of chloramphenicol residue
ترجمه فارسی عنوان
استراتژی ایمن سازی مبتنی بر نانوکاتالیست طلا همراه با کاهش کاتالیزوری 4-نیتروفنول برای نظارت حساس بر روی کلرامفنیکول باقی مانده
کلمات کلیدی
کلارفنیکول، مواد غذایی، نانوکاتالیست طلا، ایمونوآسیا، طیف سنجی اشعه ماوراء بنفش
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• We report a new competitive-type immunoassay protocol for the detection of antibiotic residue.
• Gold nanoparticles are used as nanotags and nanocatalysts for signal amplification.
• The assay is implemented by catalytic reduction of gold nanocatalysts toward 4-nitropheneol.

A new competitive-type immunosensing system based on gold nanoparticles toward catalytic reduction of 4-nitrophenol (4-NP) was developed for sensitive monitoring of antibiotic residue (chloramphenicol, CAP, used in this case) by using ultraviolet–visible (UV–vis) spectrometry. Gold nanoparticle (AuNP) with 16 nm in diameter was initially synthesized and functionalized with CAP–bovine serum albumin (CAP–BSA) conjugate, which were used as the competitor on monoclonal anti-CAP antibody-coated polystyrene microtiter plate (MTP). In the presence of target CAP, the labeled CAP–BSA on the AuNP competed with target CAP for the immobilized antibody on the MTP. The conjugated amount of CAP–BSA–AuNP on the MTP decreased with the increase of target CAP in the sample. Upon addition of 4-NP and NaBH4 into the MTP, the carried AuNP could catalytically reduce 4-NP to 4-aminophenol (4-AP), and the as-produced 4-AP could be monitored by using UV–vis absorption spectroscopy. Experimental results indicated that the absorbance at 403 nm increased with the increment of target CAP concentration in the sample, and exhibited a dynamic range from 0.1 to 100 ng mL−1 with a detection limit (LOD) of 0.03 ng mL−1 at the 3sblank level. Intra- and inter-assay coefficients of variation were lower than 5.5% and 8.0%, respectively. In addition, the methodology was evaluated for CAP spiked honey and milk samples, respectively. The recovery was 92–112%.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 830, 9 June 2014, Pages 42–48
نویسندگان
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