Development of an impedimetric immunosensor for the determination of 3-amino-2-oxazolidone residue in food samples

► A new method for AOZ detection was firstly reported based on immobilizing the self-made AOZ-McAb on the Au-SAMs by the impedimetric immunosensors. ► The proposed immunosensor exhibited high sensitivity, wide linear range, acceptable reproducibility and selectivity. It provided a simple method for direct detection of AOZ residue in food samples. ► The results were in good agreement with what was obtained by HPLC-MS/MS. Compared to HPLC-MS/MS, the proposed method can shorten the whole analysis time and simplify the sample's treatment.