کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1166983 1491137 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzyme-linked immunosensor based on super paramagnetic nanobeads for easy and rapid detection of okadaic acid
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Enzyme-linked immunosensor based on super paramagnetic nanobeads for easy and rapid detection of okadaic acid
چکیده انگلیسی

Okadaic acid (OA), a lipophilic phycotoxin highly toxic to humans is produced by toxigenic dinoflagellates. The need to develop high performing methods for OA analysis able to improve the traditional ones is evident. In this work, competitive indirect enzyme-linked electrochemical immunosensor based on super paramagnetic nanobeads has been developed for the detection of OA. Streptavidin-coated magnetic beads were used as support to immobilize the biotinylated OA. Preliminary, colorimetric tests were performed in order to optimize different experimental parameters. Electrochemical detection was carried out by differential pulse voltammetry (DPV). The limit of detection (LOD) (0.38 μg L−1), the mid point value (IC50) (3.15 μg L−1) and the time needed (60 min) for analysis of a real sample validated the developed electrochemical immunosensor as a promising tool for routine use. The matrix effect and the recovery rate were also assessed, showing an excellent percentage of recovery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 690, Issue 2, 1 April 2011, Pages 248–252
نویسندگان
, , ,