کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1168477 1491159 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid and simple method for ultrasensitive electrochemical immunoassay of protein by an electric field-driven strategy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A rapid and simple method for ultrasensitive electrochemical immunoassay of protein by an electric field-driven strategy
چکیده انگلیسی

A sensitive, rapid and simple method for analysis of protein was proposed by introducing an electric field-driven technique to the incubation process of immunoassay and immobilizing horseradish peroxidase (HRP) labeled antibody in a newly designed gel matrix to construct an immunosensor. With an electric field-driven technique, the incubation for immuno-recognition could be completed within 2 min. The immobilized HRP showed excellent direct electrochemistry in this new gel matrix, and the detection procedure was greatly simplified by directly monitoring the sensitive electrochemical signal of HRP upon the immunoreaction. Using α-fetoprotein as a model the linear detection range was from 0.02 to 2.0 ng mL−1 with a detection limit down to 138 amol mL−1. The immunosensor and the proposed detection method showed good specificity and acceptable stability and accuracy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 644, Issues 1–2, 30 June 2009, Pages 36–41
نویسندگان
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