کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1171090 1491174 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Extraction and determination of papaverin in pericarpium papaveris using aqueous two-phase system of poly(ethylene glycol)–(NH4)2SO4 coupled with high-performance liquid chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Extraction and determination of papaverin in pericarpium papaveris using aqueous two-phase system of poly(ethylene glycol)–(NH4)2SO4 coupled with high-performance liquid chromatography
چکیده انگلیسی

Based on aqueous two-phase system (ATPS) of poly(ethylene glycol) (PEG)–(NH4)2SO4, a simple pretreatment approach was developed for the extraction and determination of papaverin in pericarpium papaveris. The influence factors on phase behavior of the ATPS and partition behavior of papaverin was investigated, and partition mechanism based on the hydrophobic interaction between PEG and analyte molecules was proposed. Under the optimal conditions, the extraction efficiencies for papaverin were 93–96%, and the recoveries of the added standard were 97–106% with relative standard deviations of 1.8–2.5%. Combined with a high-performance liquid chromatography (HPLC) method, this extraction technique has been successfully applied to the determination of papaverin in pericarpium papaveris with the detection limit of 2 ng mL−1 and the linear range of 0.10–10 μg mL−1. Compared with the conventional liquid–liquid extraction or solid-phase extraction, this method was more environmentally benign, more cost effective and much simpler due to the direct injection of the upper phase into HPLC system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 590, Issue 2, 8 May 2007, Pages 187–194
نویسندگان
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