کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1172108 | 960746 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of isoproturon at trace level by solid phase competitive fluoroimmunosensing after enrichment in a sol-gel immunosorbent
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Isoproturon was extracted selectively from environmental materials (water samples) using an immunosorbent column containing anti-isoproturon antibodies encapsulated in a silica matrix by a sol-gel process. A phosphate buffered saline (PBS) conditioned immunosorbent column was used to on-line preconcentrate 5 ml well and tap water containing 0.05 μg lâ1 of isoproturon, which were desorbed with 75 μl of citric acid and determined with a solid phase competitive fluoroimmunoassay. The solid phase of the immunosensor, consisting of a sol-gel glass doped with anti-isoproturon monoclonal antibody, was placed on the flow-cell of the spectrofluorometer. Free isoproturon in solution competed with a fluorescent conjugated isoproturon and reduced the support bonded fluorescence in a concentration-dependent manner. The on-line method has a detection limit of 9.7 ng lâ1, relative standard deviation of 4 and 3% for 0.05 and 0.5 μg lâ1, respectively, and recoveries higher than 90% for tap and well water. For comparison the off-line extraction and clean up using a C18 cartridge is also reported.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 562, Issue 1, 9 March 2006, Pages 122-127
Journal: Analytica Chimica Acta - Volume 562, Issue 1, 9 March 2006, Pages 122-127
نویسندگان
P. Pulido-Tofiño, J.M. Barrero-Moreno, M.C. Pérez-Conde,