کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1172621 | 960796 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A multicommuted fluorescence-based sensing system for simultaneous determination of Vitamins B2 and B6
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A multicommuted fluorescence-based sensing system for simultaneous determination of Vitamins B2 and B6 A multicommuted fluorescence-based sensing system for simultaneous determination of Vitamins B2 and B6](/preview/png/1172621.png)
چکیده انگلیسی
A multicommuted flow-through optosensor based on the direct fluorescence measurements of Vitamins B2 and B6 using a non-polar sorbent (C18 silica gel) as solid sensing zone (to accomplish the separation and subsequent preconcentration/detection of the target analytes) have been developed. The proposed flow system was controlled by Java-written home-made software and designed using three-way solenoid valves for independent automated manipulation of sample and carrier solutions. The native fluorescence signal was simultaneously monitored at two pairs of excitation/emission wavelengths (450/519 and 294/395 for B2 and B6, respectively). The separation of the analytes was performed in the detection flow cell, using the differences in the sorption/elution process on the solid support between the two vitamins, due to their different polarity. Using an optimised sampling time, the analytical signal showed linearity in the range 0.01-0.4 and 0.15-3 μg mlâ1 with detection limits of 0.003 and 0.045 μg mlâ1 for B2 and B6, respectively, obtaining R.S.D. (%) values better than 2% for both analytes. The proposed methodology was applied to different pharmaceutical preparations, obtaining remarkably good results with recoveries ranging from 96 to 107.5%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 555, Issue 1, 5 January 2006, Pages 128-133
Journal: Analytica Chimica Acta - Volume 555, Issue 1, 5 January 2006, Pages 128-133
نویسندگان
Eulogio J. Llorent-MartÃnez, Juan F. GarcÃa-Reyes, Pilar Ortega-Barrales, Antonio Molina-DÃaz,