Time resolved thermodynamics associated with ligand photorelease in heme peroxidases and globins: Open access channels versus gated ligand release

► The photothermal summary demonstrate unique dynamics for heme ligand migration. ► Peroxidases with large channels are dynamically consistent with fast ligand exit. ► Globin dynamics reveal more complex conformational regulation for ligand release. ► Globin dynamics are unique to each type of protein within the class. ► Photothermal methods provide new insights into heme ligand dynamics.