کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182787 1491646 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of Temperature Profile on Growth and Proliferation of Myoblasts on ITO Glass Chips
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Impact of Temperature Profile on Growth and Proliferation of Myoblasts on ITO Glass Chips
چکیده انگلیسی

To study the impact of different temperatures on the growth and proliferation of myoblasts, specific electric fields were loaded on indium tin oxide (ITO) glass chips to generate temperature profiles. In several zones with different temperatures (38, 39, 40 and 41 °C), microchambers with equal size were fabricated for the culture of mouse myoblasts. Impacts of temperatures on growth and proliferation of myoblasts were studied by stimulating cells for continuous 5 days (30 min/day). Microscopic observation of cell morphology and flow cytometric detection results showed that certain thermal stimulation could promote the proliferation of myoblasts. The number of cells was obviously increased after stimulation at 40 °C. For equal total stimulation time, short-term and multiple thermal stimulation could achieve better effect, and maximum proliferation index could reach 38.39.

Specific electric fields were loaded on ITO glass chips to generate temperature profiles to study their impact on the growth and proliferation of myoblasts. Cells were stimulated for continuous 5 days, and the results showed that certain thermal stimulation could promote the proliferation of myoblasts. Short-term and multiple thermal stimulation could achieve better effect.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 41, Issue 8, August 2013, Pages 1171–1176
نویسندگان
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