کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1186951 | 963452 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of two new sensitive ELISAs for Hesperetin and Naringenin in biological fluids
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
IC50matrix assisted laser desorption ionisationN-bromosuccinimideNBSppmTLCDMFPBSnuclear magnetic resonance - رزونانس مغناطیسی هستهایBSA - BSADMSO - DMSON,N-dimethylformamide - N، N-dimethylformamidebovine serum albumin - آلبومین سرم گاوPolyclonal antibodies - آنتیبادیهای پلی کلنالenzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استUrine - ادرار part per million - بخشی در میلیونELISA - تست الیزاNMR - تشدید مغناطیسی هستهای Thyroglobulin - تیروگلوبولینDimethyl sulfoxide - دیمتیل سولفواکسیدInfrared spectroscopy - طیف سنجی مادون قرمزConcentration for 50% Inhibition - غلظت 50٪ مهارphosphate buffer saline - فسفات بافر شورFlavanone - فلاونونMALDI - مالدیNaringenin - نایرینگینینHaptens - هاپنزHesperetin - هسپرتینPlasma - پلاسماoptical density - چگالی نوریthin layer chromatography - کروماتوگرافی لایه نازک
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development and validation of two new sensitive ELISAs for Hesperetin and Naringenin in biological fluids Development and validation of two new sensitive ELISAs for Hesperetin and Naringenin in biological fluids](/preview/png/1186951.png)
چکیده انگلیسی
Carboxylic acid haptens were synthesised, for Hesperetin (Hesp) and Naringenin (Nar). They had a spacer arm (4 or 6 carbons long) on the C7 position. Haptens were coupled to bovine serum albumin. Polyclonal antibodies were generated. Enzyme-linked immunosorbent assays (ELISAs) were developed. Owing to sensitivity, one antibody for each flavanone was retained, namely anti-h4-Hesp and anti-h4-Nar. For Hesp and Nar, IC50 of the standard curves were 1.29Â pmol/well and 0.72Â pmol/well, respectively. Intra-assay and inter-assay variations were 10.24% and 10.53%, respectively for Hesp and 10.03% and 10.79%, respectively for Nar. Anti-h4-Hesp was highly specific when anti-h4-Nar showed cross-reactions with liquiritigenin and eriodictyol, which were significantly reduced in heterologous assay conditions. Assays were validated by comparisons with HPLC Coularray. Measurements were done on diet, plasma and urine samples from mice fed on diets enriched with Hesp or Nar and on a range of diluted mice urine samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 118, Issue 2, 15 January 2010, Pages 472-481
Journal: Food Chemistry - Volume 118, Issue 2, 15 January 2010, Pages 472-481
نویسندگان
Svitlana Shinkaruk, Valérie Lamothe, Jean-Marie Schmitter, Claudine Manach, Christine Morand, Annie Berard, Bernard Bennetau, Catherine Bennetau-Pelissero,