کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1198769 1493485 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of the ultraviolet–visible photoproducts of thiophanate-methyl using high performance liquid chromatography coupled with high resolution tandem mass spectrometry—Detection in grapes and tomatoes
ترجمه فارسی عنوان
تشخیص نوتروپانیت ملی با استفاده از کروماتوگرافی مایع با کارایی بالا همراه با اسپکترومتریای توزیع با وضوح بالا و تشخیص در محصولات انگور و گوجه فرنگی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• UV–visible irradiation of thiophanate-methyl (TM) led to nine photoproducts.
• HPLC coupled with ultra-high resolution FTICR-MS allowed byproducts characterization.
• TM and one photoproduct were detected in peel and flesh of fruits treated with TM.
• In vitro tests showed that irradiation increases the global ecotoxicity of TM.
• In silico tests permitted to attribute the ecotoxicity enhancement to a photoproduct.

UV–visible irradiation of thiophanate-methyl (TM) led to the formation of nine photoproducts that were characterized by high performance liquid chromatography coupled with high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). Although carbendazime has been reported in the literature to be the major metabolite and photoproduct of thiophanate-methyl, it was not detected in this study. However, an isomer of carbendazime referred as PP2, which was unambiguously characterized owing to CID experiments, was found in great abundance. Grape berries and cherry tomatoes treated with aqueous solutions of thiophanate-methyl were submitted to irradiation under laboratory conditions. TM and PP2 were detected in both peel and flesh of berries. The ability of TM and PP2 to pass through the fruit skin has been shown to be highly compound and matrix dependent. In vitro bioassays on Vibrio fischeri bacteria showed that the global ecotoxicity of the TM solution increases significantly with the irradiation time. PP2 should likely contribute to this ecotoxicity enhancement since in silico estimations for Daphnia magna provide a LC50 value seven times lower for PP2 than for the parent molecule.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1441, 8 April 2016, Pages 75–82
نویسندگان
, , , , , , , ,