کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11987981 | 1051594 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice
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کلمات کلیدی
STAT3CRISPRDSSCRISPR-Cas9AOMazoxymethaneJanus kinase - کیناز جانوس STAT - آمارinterleukin - اینترلوکینInterleukin 11 - اینترلوکین 11clustered regularly interspaced short palindromic repeats - به طور منظم تکرار پینگندرومی کوتاه مدت میان دو طرف تقسیم می شودProliferation - ترویجDextran sulfate sodium - سولفات سدیم دکسترانSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیKnockout mice - موش نابود شدهJAK - چگونه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Interleukin (IL)-11 belongs to the members of the IL-6 family of cytokines and is involved in a variety of biological responses, including hematopoiesis, bone development, and carcinogenesis. However, the cellular sources of IL-11 and regulation of IL-11 expression under physiological and pathological conditions are not fully understood. One of the causes to prevent characterization of IL-11 in vivo is due to the lack of reliable antibodies that detect IL-11 by immunohistochemistry. Moreover, although mice lacking Il11ra have been generated and extensively characterized, Il11-deficient mice have not been characterized yet. Here we generated two anti-IL-11 antibodies that blocked biological activities of IL-11 and detected IL-11 by immunohistochemistry, respectively. One clone of anti-IL-11 antibodies blocked IL-11-, but not IL-6-induced cell proliferation and IL-11-induced phosphorylation of STAT3 of an IL-11-dependent cell line. Moreover, we used recently established Il11-deficient mice to test the specificity of anti-IL-11 antibodies for immunohistochemistry. Another clone of anti-IL-11 antibodies stained stromal cells surrounding tumors of the colon of wild-type, but not Il11-deficient mice following treatment with Azoxymethane plus dextran sulfate sodium. Together, these newly developed anti-IL-11 antibodies provide a better understanding of the functions of IL-11 in vivo under various physiological and pathological conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 505, Issue 2, 28 October 2018, Pages 453-459
Journal: Biochemical and Biophysical Research Communications - Volume 505, Issue 2, 28 October 2018, Pages 453-459
نویسندگان
Yutaka Deguchi, Takashi Nishina, Kenichi Asano, Masaki Ohmuraya, Yoshiko Nakagawa, Naomi Nakagata, Tetsushi Sakuma, Takashi Yamamoto, Kimi Araki, Tetuo Mikami, Masato Tanaka, Hiroyasu Nakano,