کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1199030 1493498 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of nano and conventional liquid chromatographic methods for the separation of (+)-catechin-ethyl-malvidin-3-glucoside diastereoisomers
ترجمه فارسی عنوان
مقایسه روشهای کروماتوگرافی مایع نانو و مرسوم برای جداسازی دیاستروئوزومرهای (+) - کاتچین - اتیل - مالیدین - 3 - گلوکوزید
کلمات کلیدی
کروماتوگرافی نانو مایع، ستون مویرگی، کروماتوگرافی مایع با کارایی بالا، آنتوسیانین ها، رنگ مضر،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Nano-liquid chromatography and conventional HPLC were used for the separation of diastereomers of (+)-catechin-ethyl-malvidin-3-glucoside. Those bridged anthocyanin dyes were obtained by reaction of (+)-catechin with malvidin-3-glucoside in the presence of acetaldehyde. Both diastereomers were isolated with semipreparative chromatography and their structures were confirmed by nuclear magnetic resonance and mass spectrometry. In-laboratory prepared capillary columns packed with fully porous particles Chromosphere C18, dp = 3 μm, core-shell particles Kinetex C18, dp = 2.6 μm (100 μm i.d.) and monolithic column Chromolith CapRod (100 μm i.d.) were used for the separation of (+)-catechin, malvidin-3-glucoside and both diastereomers. Chromosphere C18 stationary phase provided the best chromatographic performance. Mobile phase containing water:acetonitrile (80:20) acidified with trifluoroacetic acid (0.1%, v/v/v) was used in an isocratic elution mode with a flow rate of 360 nL min−1. Separation of studied compounds was achieved in less than 7 min under optimized conditions. The nano-liquid chromatographic method and a conventional HPLC one using the same fully porous particles (Chromosphere C18, 3 μm, 100 mm × 4.6 mm) were compared providing higher separation efficiency with the first analytical method and similar selectivity. A better peak symmetry and higher resolution of the studied diastereomers was achieved by conventional chromatography. Nevertheless, nano-liquid chromatography appeared to be useful for the separation of complex anthocyanin dyes and can be utilized for their analysis in plant and food micro-samples. The developed method was used for analysis of red wine grape pomace.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1428, 8 January 2016, Pages 126-133
نویسندگان
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