کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1199063 1493559 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of bronchoalveolar lavage bile acids by solid phase microextraction liquid chromatography–tandem mass spectrometry in combination with metabolite profiling: Comparison with enzymatic assay
ترجمه فارسی عنوان
تعیین اسیدهای صفراوی لارو برونکلو آلوئولار با استفاده از کروماتوگرافی مایع اکستراکتیو مایع کروماتوگرافی جامد با استفاده از طیف سنجی جرم دو طرفه در تشخیص متابولیت: در مقایسه با آنزیمی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Thin-film SPME LC-MS/MS method offers fast and reliable analysis of bronchoalveolar bile acids.
• Sample preparation time was 1.3 min/sample.
• A strong correlation was found between LC-MS-based metabolites profiling and enzymatic assay.

A thin-film solid-phase microextraction (SPME) method coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis was developed for high-throughput determination of bronchoalveolar lavage bile acids. The proposed method was validated according to the bioanalytical method validation guidelines. LOQ and LLOQ were 0.007 and 0.02 μmol/L, respectively. The accuracy and the precision were <7 and <4%, respectively. The performance of the proposed method was also compared with an optimized enzymatic cycling assay. Results showed a weak correlation between total concentration of bile acid (BAs) obtained with enzymatic assay and cumulative concentration of specific BAs determined with SPME-LC-MS/MS. This discrepancy was probably due to the presence of other BAs in bronchoalveolar lavage fluid (BALF) samples. Metabolites profiling of BALF samples extracts using a high-resolution mass spectrometer (HRMS) revealed the presence of additional BAs, which were not included in the proposed method. After considering these additional BAs, a strong correlation was found between the LC-MS method and the enzymatic assay. Unsupervised statistical analysis conducted on HRMS data also showed clear separation within BALF samples, depending on the presence of BAs and other lipids. SPME-LC-MS-based metabolites profiling may provide additional information for diagnosis occurrence and severity of gastric reflux/aspiration in lung transplant patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1367, 7 November 2014, Pages 33–38
نویسندگان
, , , , , ,