کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1202740 1493693 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of astragalin and astragaloside content in Radix Astragali using high-performance liquid chromatography coupled with pulsed amperometric detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of astragalin and astragaloside content in Radix Astragali using high-performance liquid chromatography coupled with pulsed amperometric detection
چکیده انگلیسی

Astragalin and astragalosides were measured in Radix Astragali using reversed-phase chromatography coupled with pulsed amperometric detection. Because the target compounds showed poor stability in aqueous solutions, they were extracted in 100% methanol under reflux. All compounds were detected with high sensitivity under highly alkaline conditions using sodium hydroxide as a post-column eluent. The limits of detection and quantification of target compounds were 0.02–0.36 μg/mL and 0.06–1.09 μg/mL, respectively, and the linear regression coefficients were 0.9982–1.0000. The intra- and inter-day precisions were <0.92% in retention time and <4.78% in calculated contents. Average recoveries were >91.33%. Astragalin and astragaloside contents between Radix Astragali at different ages and in different parts were successfully determined without sample purification or concentration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1232, 6 April 2012, Pages 212–217
نویسندگان
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