کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1203444 965113 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity
چکیده انگلیسی

Biocompatible poly(ethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate) monoliths were prepared for size exclusion chromatography (SEC) of proteins in the capillary format using Brij 58P in a mixture of hexanes and dodecanol as porogens. The monolithic columns provided size separation of four proteins in 20 mM sodium phosphate buffer (pH 7.0) containing 0.15 M NaCl, and there was a linear relationship between the retention times and the logarithmic values of the molecular weights. Compared to SEC monoliths previously synthesized using a triblock copolymer of polyethylene oxide and polypropylene oxide, an increase in mesoporosity was confirmed by inverse size exclusion chromatography. As a result, improved protein separation in the high molecular weight range and reduced column back-pressure were observed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1217, Issue 52, 24 December 2010, Pages 8181–8185
نویسندگان
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