کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1203518 965116 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Annealing of silica to reduce the concentration of isolated silanols and peak tailing in reverse phase liquid chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Annealing of silica to reduce the concentration of isolated silanols and peak tailing in reverse phase liquid chromatography
چکیده انگلیسی

Non-porous, colloidal silica particles were annealed at three different temperatures, 800, 900 and 1050 °C. The adsorption of lysozyme, a probe of surface roughness, was consistent with progressively reduced surface roughness as temperature increased. The heat treated silica particles were rehydroxylated and then used to pack UHPLC columns. The cationic protein lysozyme was used to probe silanol activity, which exhibited progressively less tailing as the annealing temperature increased. FTIR spectroscopy confirmed that the abundance of isolated silanols on the surface was reduced by annealing at 900 °C or 1050 °C. FTIR also revealed that there was markedly increased hydrogen bonding of the isolated silanols to neighbors after rehydroxylation. These results combine to support the hypothesis that (a) isolated silanols on silica cause tailing in RP-LC and (b) nonplanar topography gives rise to isolated silanols.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 31, 5 August 2011, Pages 5131–5135
نویسندگان
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