کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1203655 965120 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolite profiling on apple volatile content based on solid phase microextraction and gas-chromatography time of flight mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Metabolite profiling on apple volatile content based on solid phase microextraction and gas-chromatography time of flight mass spectrometry
چکیده انگلیسی

A headspace SPME GC–TOF-MS method was developed for the acquisition of metabolite profiles of apple volatiles. As a first step, an experimental design was applied to find out the most appropriate conditions for the extraction of apple volatile compounds by SPME. The selected SPME method was applied in profiling of four different apple varieties by GC–EI-TOF-MS. Full scan GC–MS data were processed by MarkerLynx software for peak picking, normalisation, alignment and feature extraction. Advanced chemometric/statistical techniques (PCA and PLS-DA) were used to explore data and extract useful information. Characteristic markers of each variety were successively identified using the NIST library thus providing useful information for variety classification. The developed HS-SPME sampling method is fully automated and proved useful in obtaining the fingerprint of the volatile content of the fruit. The described analytical protocol can aid in further studies of the apple metabolome.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 28, 15 July 2011, Pages 4517–4524
نویسندگان
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