کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1206639 965242 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive high-performance liquid chromatography method of non-polar ginsenosides by alkaline-enhanced pulsed amperometric detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive high-performance liquid chromatography method of non-polar ginsenosides by alkaline-enhanced pulsed amperometric detection
چکیده انگلیسی

We determined the minute amount of non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection (RP-HPLC-PAD) method. Non-polar ginsenosides efficiently extracted by ethyl acetate were well separated in 40 min using a water–acetonitrile gradient eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limits (S/N = 3) were 0.03–0.10 ng. The coefficients of linear regression were 0.9972–0.9990. Intra- and inter-day precision (RSDs) was less than 8.34% and average recovery was 98.06–102.73%. The total amount of non-polar ginsenosides in hairy root of red ginseng was slightly higher than in the main root.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 20, 15 May 2009, Pages 4445–4450
نویسندگان
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