کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206646 | 965242 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of deamidated peptide variants by micro-preparative capillary electrophoresis and mass spectrometry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization of deamidated peptide variants by micro-preparative capillary electrophoresis and mass spectrometry Characterization of deamidated peptide variants by micro-preparative capillary electrophoresis and mass spectrometry](/preview/png/1206646.png)
چکیده انگلیسی
Capillary electrophoresis (CE) was compared with reversed-phase liquid chromatography for its ability to separate native and deamidated peptides. CE is shown to provide superior resolution of these peptides due to its charge-based separation mechanism. Fraction collection performed using a standard CE instrument equipped with a 96-well plate permits subsequent characterization by nanospray mass spectrometric (MS) analysis. Additional in-depth analysis by MS/MS is able to provide the location of the deamidation site based on y-ion mass shifts of 1 Da.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 20, 15 May 2009, Pages 4499–4503
Journal: Journal of Chromatography A - Volume 1216, Issue 20, 15 May 2009, Pages 4499–4503
نویسندگان
Lynn A. Gennaro, Oscar Salas-Solano,