کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207085 965254 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of γ-hydroxybutyric acid in clinical samples using capillary electrophoresis with contactless conductivity detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of γ-hydroxybutyric acid in clinical samples using capillary electrophoresis with contactless conductivity detection
چکیده انگلیسی

A method for the determination of γ-hydroxybutyric acid (GHB) in urine and serum samples with capillary electrophoresis using capacitively coupled contactless conductivity detection (CE-C4D) was developed. The optimized separation buffer consisting of 20 mM of arginine, 10 mM of maleic acid and 30 μM of cetyltrimethylammonium bromide (CTAB) contained 5 mM vancomycin to facilitate the separation of γ-hydroxybutyric acid from β-hydroxybutyric acid (BHB), which is also present in clinical samples. The detection limits in the clinical samples were found to be about 2 μg/ml, which is well below the required sensitivity for the recognition of overdosage and adequate for the determination of endogenous concentrations in urine. The determination of GHB in both types of samples was carried out directly after a fourfold dilution without requiring any derivatization or extraction procedures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1213, Issue 1, 5 December 2008, Pages 100–104
نویسندگان
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