کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1207717 | 965278 | 2007 | 6 صفحه PDF | دانلود رایگان |

Poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths have been synthetized in fused-silica capillary. The monomer mixture composition, initiation mode and porogen composition were optimized in order to provide a monolith with an homogeneous morphology and able to generate an electroosmotic flow via the incorporation of a small percentage of monomers possessing sulfonate group. Anti-ochratoxin A antibodies were immobilized through a single step on the epoxy groups leading to a miniaturized immunoextraction column. In order to evaluate the specificity of the analyte–antigen interaction on this immunosorbent, the retention of ochratoxin A was examined on this support but also on two complementary sorbents: one constituted by the non-bonded monolith and another one bonded with non-specific antibodies. Only the monolith bonded with anti-ochratoxin A antibodies lead to retention, showing the specificity of the interactions involved. This affinity phase based on a monolithic polymer support exhibits a high potential for specific preconcentration of small molecules.
Journal: Journal of Chromatography A - Volume 1149, Issue 2, 18 May 2007, Pages 145–150