کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1207877 | 1493723 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of Diol column under adsorption and mixed partition-adsorption normal-phase liquid chromatography mode for the separation of purines and pyrimidines
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The diol-bonded phase (column LiChrospher 100 Diol) has been studied for the separation of some purines and pyrimidines under normal-phase liquid chromatography (NPLC) conditions. Retention time, column efficiency, and selectivity of column with diol-phase were compared to those of unmodified silica (column LiChrospher SI-60). It was established that under adsorption NPLC mode application of diol-phase can reduce the separation time and a little improve the column efficiency. Significant improvement of the column efficiency for polar solutes is observed if mixed partition-adsorption NPLC mode is used. The investigation has shown that application of diol-phase instead of bare silica is useful not only under adsorption, but also under mixed partition-adsorption mode if the system with specific selectivity is necessary.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1189, Issues 1â2, 2 May 2008, Pages 52-58
Journal: Journal of Chromatography A - Volume 1189, Issues 1â2, 2 May 2008, Pages 52-58
نویسندگان
H. Kažoka,