کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1208549 1493748 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Elution of tightly bound solutes from concanavalin A Sepharose: Factors affecting the desorption of cottonmouth venom glycoproteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Elution of tightly bound solutes from concanavalin A Sepharose: Factors affecting the desorption of cottonmouth venom glycoproteins
چکیده انگلیسی

Some glycoproteins bind so tightly to concanavalin A Sepharose that common desorption techniques are ineffective, so a systematic exploration of factors affecting desorption of cottonmouth venom glycoproteins was undertaken. Glycoprotein desorption is greatly improved by introducing up to four pauses of 5–10 min duration into the elution step. Eluent concentrations above 250 mM methylglucoside or 500 mM methyl-mannoside reduced glycoprotein desorption. Eluent NaCl diminished glycoprotein desorption. Most venom glycoproteins desorb more readily as pH diminishes from 6.0 to 4.0, but phosphodiesterase shows the opposite pattern. Eluents recommended by the supplier for desorbing solutes or for column cleaning were ineffectual.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1154, Issues 1–2, 22 June 2007, Pages 308–318
نویسندگان
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