کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1208555 1493748 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of procaine metabolism as probe for the butyrylcholinesterase enzyme investigation by simultaneous determination of procaine and its metabolite using capillary electrophoresis with electrochemiluminescence detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Characterization of procaine metabolism as probe for the butyrylcholinesterase enzyme investigation by simultaneous determination of procaine and its metabolite using capillary electrophoresis with electrochemiluminescence detection
چکیده انگلیسی

Capillary electrophoresis with electrochemiluminescene detection was used to characterize procaine hydrolysis as a probe for butyrylcholinesterase by in vitro procaine metabolism in plasma with butyrylcholinesterase acting as bioscavenger. Procaine and its metabolite N,N-diethylethanolamine were separated at 16 kV and then detected at 1.25 V in the presence of 5.0 mM Ru(bpy)32+, with the detection limits of 2.4 × 10−7 and 2.0 × 10−8 mol/L (S/N = 3), respectively. The Michaelis constant Km value was 1.73 × 10−4 mol/L and the maximum velocity Vmax was 1.62 × 10−6 mol/L/min. Acetylcholine bromide and choline chloride presented inhibition effects on the enzymatic cleavage of procaine, with the 50% inhibition concentration (IC50) of 6.24 × 10−3 and 2.94 × 10−4 mol/L.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1154, Issues 1–2, 22 June 2007, Pages 368–372
نویسندگان
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