کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1208555 | 1493748 | 2007 | 5 صفحه PDF | دانلود رایگان |

Capillary electrophoresis with electrochemiluminescene detection was used to characterize procaine hydrolysis as a probe for butyrylcholinesterase by in vitro procaine metabolism in plasma with butyrylcholinesterase acting as bioscavenger. Procaine and its metabolite N,N-diethylethanolamine were separated at 16 kV and then detected at 1.25 V in the presence of 5.0 mM Ru(bpy)32+, with the detection limits of 2.4 × 10−7 and 2.0 × 10−8 mol/L (S/N = 3), respectively. The Michaelis constant Km value was 1.73 × 10−4 mol/L and the maximum velocity Vmax was 1.62 × 10−6 mol/L/min. Acetylcholine bromide and choline chloride presented inhibition effects on the enzymatic cleavage of procaine, with the 50% inhibition concentration (IC50) of 6.24 × 10−3 and 2.94 × 10−4 mol/L.
Journal: Journal of Chromatography A - Volume 1154, Issues 1–2, 22 June 2007, Pages 368–372