کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1210113 1493776 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Experimental design for a basic mixture on a fluorinated packing: The effect of composition of the mobile phase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Experimental design for a basic mixture on a fluorinated packing: The effect of composition of the mobile phase
چکیده انگلیسی

An optimization methodology is introduced for investigating the separation and the retention behavior of analytes on a new fluorinated reversed-phase packing. Ten basic compounds were selected as test probes to study the predictive models developed by using SPSS and MATLAB software. A two-level orthogonal array design (OAD) was used to extract significant parameters.The significant factors were optimised using a central composite design to obtain the quadratic relationship between the dependent and the independent variables. Using this strategy, response surfaces were derived as the 3D and contour plots, and mathematical models were defined for the separation. The models had a satisfactory coefficient (R2 > 0.97, n = 16). For the test compounds, the best separation condition was: MeCN/30 mM phosphate buffer pH 7.1(55.5:44.5, v/v) and 10 basic solutes were resolved in 22 min. The significant influence of the concentration of buffer shows that different mechanisms of separation for basic compounds on the fluorinated packing exist compared with a common ODS stationary phase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1105, Issues 1–2, 10 February 2006, Pages 77–86
نویسندگان
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