کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1211452 | 1493762 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Retention in overloaded columns, an experimental approach Retention in overloaded columns, an experimental approach](/preview/png/1211452.png)
Employing a micro-bore silica capillary coated with Carbowax 20 M, the dependence of chromatographic retention upon operative variables was studied surpassing the sample capacity of the column. Solution thermodynamics in the non-linear range of the absorption isotherm of n-alkanes on poly(ethylene oxide) were analyzed interpreting the experimental data through a retention equation deduced in a preceding theoretical work. At 120 °C, and pressures up to 11 bar abs, deviations from the ideal-gas behavior are found to be negligible, either for the fluid dynamics of the carrier-gas, or the thermodynamics of solution of the n-alkanes. Within the experimental error, for all practical purposes the mobile phase can be treated as an ideal gas. This constraint allows studying a solute molecule placed in an environment ranging from solvent monomers only, to a mixture of varying composition of solvent and solute, avoiding effects from significant interactions in the gas phase. In the experimental conditions explored, the absorption isotherm can be represented by taking only two-terms of its power series development.
Journal: Journal of Chromatography A - Volume 1128, Issues 1–2, 22 September 2006, Pages 203–207