کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1215085 | 1494168 | 2008 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of rimonabant in human plasma and hair by liquid chromatography–mass spectrometry Determination of rimonabant in human plasma and hair by liquid chromatography–mass spectrometry](/preview/png/1215085.png)
Rimonabant is the first therapeutically relevant cannabinoid antagonist, licensed in Europe for treatment of obesity when a risk factor is associated. The objective of this study was to develop and validate a method for measurement of rimonabant in human plasma and hair using liquid chromatography coupled to mass spectrometry (LC–MS/MS). Rimonabant and AM-251 (internal standard) were extracted from 50 μL of plasma or 10 mg of hair using diethylether. Chromatography was performed on a 150 mm × 2.1 mm C18 column using a mobile phase constituted of formate buffer/acetonitrile. Rimonabant was ionized by electrospray in positive mode, followed by detection with mass spectrometry. Data were collected either in full-scan MS or in full-scan MS/MS mode, selecting the ion m/z 463.1 for rimonabant and m/z 555.1 for IS. The most intense product ion of rimonabant (m/z 380.9) and IS (m/z 472.8) were used for quantification. Calibration curves covered a range from 2.5 (lower limit of quantification) to 1000.0 ng/mL (upper limit of quantification) in plasma and from 2.5 to 1000.0 pg/mg in hair. Validation results demonstrated that rimonabant could be accurately and precisely quantified in both matrixes: accuracy and precision were within 85–115% and within 15% of standard deviation, respectively. Stability studies in plasma showed that rimonabant was stable during the assay procedure, but a 30% decrease was observed for one concentration after 3 weeks at −20 °C. This simple and robust LC–MS/MS method can be used for measuring rimonabant concentrations in human plasma and hair either in clinical or in forensic toxicology.
Journal: Journal of Chromatography B - Volume 872, Issues 1–2, 1 September 2008, Pages 115–120