کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1216353 | 967008 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat](/preview/png/1216353.png)
چکیده انگلیسی
The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN® Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issue 10, 1 April 2009, Pages 975–982
Journal: Journal of Chromatography B - Volume 877, Issue 10, 1 April 2009, Pages 975–982
نویسندگان
Hetty C. van den Broeck, Antoine H.P. America, Marinus J.M. Smulders, Dirk Bosch, Rob J. Hamer, Ludovicus J.W.J. Gilissen, Ingrid M. van der Meer,