کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217601 | 967122 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood](/preview/png/1217601.png)
چکیده انگلیسی
Plasma aromatic and sulfur containing amino acids are good indicators of protein anabolism/catabolism, while blood reduced and oxidized glutathione reflect oxidative status in an organism. Using a full factorial design for screening important variables (pH, concentration, temperature) we developed a capillary zone electrophoresis method permitting their measurements in the single run, without any derivatization procedures. The best separations were obtained within less than 30 min employing a 10 mmol/l phosphate buffer, pH 2.8, 18 °C, 15 kV voltage. Fairly good precision with a linear relationship between peak area and concentrations (r = 0.995–0.999) were obtained. The method was used to analyze human capillary blood.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 873, Issue 1, 15 September 2008, Pages 70–76
Journal: Journal of Chromatography B - Volume 873, Issue 1, 15 September 2008, Pages 70–76
نویسندگان
Gordana Žunić, Slavica Spasić,