کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217601 967122 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood
چکیده انگلیسی

Plasma aromatic and sulfur containing amino acids are good indicators of protein anabolism/catabolism, while blood reduced and oxidized glutathione reflect oxidative status in an organism. Using a full factorial design for screening important variables (pH, concentration, temperature) we developed a capillary zone electrophoresis method permitting their measurements in the single run, without any derivatization procedures. The best separations were obtained within less than 30 min employing a 10 mmol/l phosphate buffer, pH 2.8, 18 °C, 15 kV voltage. Fairly good precision with a linear relationship between peak area and concentrations (r = 0.995–0.999) were obtained. The method was used to analyze human capillary blood.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 873, Issue 1, 15 September 2008, Pages 70–76
نویسندگان
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