کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217646 1494175 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles
چکیده انگلیسی

A partial pseudo-ternary phase diagram has been studied for the cethyltrimethylammonium bromide/isooctane:hexanol:butanol/potassium phosphate buffer system, where the two-phase diagram consisting of the reverse micelle phase (L2) in equilibrium with the solvent is indicated. Based on these diagrams two-phase systems of reverse micelles were prepared with different compositions of the compounds and used for extraction and recovery of two enzymes, and the percentage of enzyme recovery yield monitored. The enzymes glucose-6-phosphate dehydrogenase (G6PD) and xylose redutase (XR) obtained from Candida guilliermondii yeast were used in the extraction procedures. The recovery yield data indicate that micelles having different composition give selective extraction of enzymes. The method can thus be used to optimize enzyme extraction processes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 862, Issues 1–2, 1 February 2008, Pages 58–63
نویسندگان
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