کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1222109 1494673 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Subcritical water extraction of alkaloids in Sophora flavescens Ait. and determination by capillary electrophoresis with field-amplified sample stacking
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Subcritical water extraction of alkaloids in Sophora flavescens Ait. and determination by capillary electrophoresis with field-amplified sample stacking
چکیده انگلیسی

The extraction and determination of cytisine, sophocarpine, matrine, sophoridine and oxymatrine in Sophora flavescens Ait. were performed using subcritical water extraction and capillary electrophoresis with field-amplified sample stacking. The effects of extraction temperature, pressure, time and cycle number on the extraction yields were investigated systematically for accelerated solvent extraction with ethanol (ASE) and accelerated solvent extraction with water (subcritical water extraction, SWE). The extraction yields obtained using SWE, ASE, water ultrasonic extraction and chloroform soaking extraction methods were compared. The electrophoresis separation buffer was monosodium phosphate (pH 3.0; 110 mM)-isopropanol (85:15, v/v). The effect of phosphoric acid added to the sample matrix on the reproducibility of the peak heights of the analytes was also examined. Cytisine, sophoridine and oxymatrine showed good linearity (R2 > 0.999) within 0.125–4.0 μg/mL, and sophocarpine and matrine exhibited good linearity (R2 > 0.998) within 0.0625–2.0 μg/mL, with the detection limits in the range of 0.004–0.0013 μg/mL. The five alkaloid contents in medicinal plants from different sources and Sophora instant granule were determined and compared.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 58, 25 January 2012, Pages 146–151
نویسندگان
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