کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1222358 967861 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separation and on-line preconcentration by stacking and sweeping of charged analytes in the plant by microemulsion electrokinetic chromatography with nonionic surfactants
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Separation and on-line preconcentration by stacking and sweeping of charged analytes in the plant by microemulsion electrokinetic chromatography with nonionic surfactants
چکیده انگلیسی

A novel on-line technique for stacking and sweeping of long sample plugs with simultaneous determination of charged analytes in the plant (protocatechuic aldehyde, rosmarinic acid, danshensu, salvianolic acid B, and protocatechuic acid) by the nonionic microemulsion electrokinetic chromatography (MEEKC) is presented. The preconcentration efficiency provided about 9–28-fold for stacking and 7–14-fold for sweeping in the enhancements of LOD. The effects of oil phase, Brij-35 and buffer concentrations on stacking and sweeping efficiency were examined in order to optimize the two methods. In nonionic MEEKC, the effect of the type of oil and buffer contents on preconcentration mechanism is often sophisticated. This study had demonstrated that the oil type and buffer content in nonionic microemulsion indeed markedly altered the affinity of microemulsion with analytes. Finally, in comparison to the stacking method, the most apparent disadvantages of the sweeping method were the relatively high limits of detection and poor peak shapes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 49, Issue 2, 20 February 2009, Pages 475–480
نویسندگان
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