کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1223337 967887 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ion-trap mass spectrometry for determination of 3,5,3′-triiodo-l-thyronine and 3,5,3′,5′-tetraiodo-l-thyronine in neonatal rat cardiomyocytes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Ion-trap mass spectrometry for determination of 3,5,3′-triiodo-l-thyronine and 3,5,3′,5′-tetraiodo-l-thyronine in neonatal rat cardiomyocytes
چکیده انگلیسی

Our short report describes a method employing electrospray ion-trap mass spectrometry (MS) connected to a reversed phase (C8) HPLC system for monitoring of 3,5,3′-triiodo-l-thyronine (T3) and its precursor 3,5,3′,5′-tetraiodo-l-thyronine (T4) in neonatal rat cardiomyocytes. The experimental protocol allows simultaneous analysis of the free thyroid hormones in nanomolar concentration range and enables observation of their distribution in cultivation medium over time. The method is a useful tool for MS2 identification of T3/T4 and analysis of their uptake into mammalian cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 53, Issue 3, 2 November 2010, Pages 688–692
نویسندگان
, , , , ,